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rsv b1  (ATCC)


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    ATCC rsv b1
    Growth kinetics of parental TPP RSV-A2 and B1 and the derived MARMs. HEp-2 cells were inoculated at an MOI of 0.01 TCID 50 /cell. The cultured supernatant was sampled twice daily for four days and viral growth was assessed using a TCID 50 assay. RSV-A2 MARMs ( A-C ) <t>and</t> <t>RSV-B1</t> MARMs ( D-F ) show similar growth to their parental TPP strains, with the exception of RSM-3 ( D ), which shows delayed and lower titre growth. MARMs, monoclonal antibody resistant mutants; MOI, multiplicity of infection; RSV, respiratory syncytial virus; TCID 50 , half-maximum tissue culture infective dose.
    Rsv B1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rsv+b1/Human+respiratory+syncytial+virus/bio_rxiv__64898__2026__02__04__703721-158-7-4
    Average 99 stars, based on 93 article reviews
    rsv b1 - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "RSM01, an extended half-life RSV monoclonal antibody with a high barrier to resistance"

    Article Title: RSM01, an extended half-life RSV monoclonal antibody with a high barrier to resistance

    Journal: bioRxiv

    doi: 10.64898/2026.02.04.703721

    Growth kinetics of parental TPP RSV-A2 and B1 and the derived MARMs. HEp-2 cells were inoculated at an MOI of 0.01 TCID 50 /cell. The cultured supernatant was sampled twice daily for four days and viral growth was assessed using a TCID 50 assay. RSV-A2 MARMs ( A-C ) and RSV-B1 MARMs ( D-F ) show similar growth to their parental TPP strains, with the exception of RSM-3 ( D ), which shows delayed and lower titre growth. MARMs, monoclonal antibody resistant mutants; MOI, multiplicity of infection; RSV, respiratory syncytial virus; TCID 50 , half-maximum tissue culture infective dose.
    Figure Legend Snippet: Growth kinetics of parental TPP RSV-A2 and B1 and the derived MARMs. HEp-2 cells were inoculated at an MOI of 0.01 TCID 50 /cell. The cultured supernatant was sampled twice daily for four days and viral growth was assessed using a TCID 50 assay. RSV-A2 MARMs ( A-C ) and RSV-B1 MARMs ( D-F ) show similar growth to their parental TPP strains, with the exception of RSM-3 ( D ), which shows delayed and lower titre growth. MARMs, monoclonal antibody resistant mutants; MOI, multiplicity of infection; RSV, respiratory syncytial virus; TCID 50 , half-maximum tissue culture infective dose.

    Techniques Used: Derivative Assay, Cell Culture, Infection, Virus

    Related Articles

    Isolation:

    Article Title: Llama-Derived Single Domain Antibodies to Build Multivalent, Superpotent and Broadened Neutralizing Anti-Viral Molecules
    Article Snippet: .. Viral stocks of RSV Long strain (originally isolated in Baltimore, MD, 1956) and RSV B1 (ATCC VR-1580) were prepared in HEp-2 cells and subsequently titrated to determine the optimal infectious dose (1–3 pfu/cell) for use in the micro neutralization assay. ..

    Neutralization:

    Article Title: Llama-Derived Single Domain Antibodies to Build Multivalent, Superpotent and Broadened Neutralizing Anti-Viral Molecules
    Article Snippet: .. Viral stocks of RSV Long strain (originally isolated in Baltimore, MD, 1956) and RSV B1 (ATCC VR-1580) were prepared in HEp-2 cells and subsequently titrated to determine the optimal infectious dose (1–3 pfu/cell) for use in the micro neutralization assay. ..

    Purification:

    Article Title: RSM01, an extended half-life RSV monoclonal antibody with a high barrier to resistance
    Article Snippet: HEp-2 cells (ATCC CCL-23) were passaged in DMEM (Gibco, high glucose, Glutamax, pyruvate, 31966-021) supplemented with 5% fetal bovine serum and pen/strep. .. Master stocks of RSV-A2 (ATCC VR-1540) and RSV-B1 (B WV/14617/85, VR-1400) were triple plaque purified in HEp-2 cells and propagated for a total of 5 and 8 passages, respectively, before use as parental strains. .. Four mAbs were evaluated (Table S1): RSM01 (Gates Medical Research Institute), Palivizumab (Synagis®), research-grade Nirsevimab (MedChemExpress, HY-P99756), and research-grade Clesrovimab (MedChemExpress, HY-P99804).

    Infection:

    Article Title: Recombinant antibodies for treatment of respiratory syncytial virus infections
    Article Snippet: In Vitro Neutralization Assays j-1 Preparation of Live RSV for In Vitro Use Human laryngeal epithelial HEp-2 cells (ATCC CLL-23) were seeded in 175 cm2 flasks at 1×107 cells/flask. .. The cells were infected with either the RSV Long (ATCC number VR-26), the RSV A2 (Advanced Biotechnologies Inc., ATCC number VR-1540) the RSV B1 (ATCC number VR-1400) or the RSV B Wash/18537 (Advanced Biotechnologies Inc., ATCC number VR-1580) strain in 3 ml serum-free medium at a ratio of 0.1 pfu/cell. ..

    Article Title: Recombinant antibodies for treatment of respiratory syncytial virus infections
    Article Snippet: .. Briefly, for the FACS assay, HEp-2 cells were infected with either the RSV Long (ATCC number VR-26) strain or the RSV B1 (ATCC number VR-1400) strain in serum-free medium at a ratio of 0.1 pfu/cell for 24 (Long strain) or 48 h (B1 strain). .. Following detachment and wash the cells were dispensed in 96-well plates and incubated with dilutions (4 pM-200 μM) of the individual anti-RSV antibodies for 1 h at 37° C. The cells were fixed in 1% formaldehyde and cell surface-bound antibody was detected by incubation with goat F(ab)2 anti-human IgG-PE conjugate (Beckman Coulter) for 30 min at 4° C. For the ELISA assay, HEp-2 cells were infected with either the RSV Long strain or the RSV 18537 strain (ATCC number VR-1580) in serum-free medium at a ratio of 0.01 pfu/cell.

    FACS:

    Article Title: Recombinant antibodies for treatment of respiratory syncytial virus infections
    Article Snippet: .. Briefly, for the FACS assay, HEp-2 cells were infected with either the RSV Long (ATCC number VR-26) strain or the RSV B1 (ATCC number VR-1400) strain in serum-free medium at a ratio of 0.1 pfu/cell for 24 (Long strain) or 48 h (B1 strain). .. Following detachment and wash the cells were dispensed in 96-well plates and incubated with dilutions (4 pM-200 μM) of the individual anti-RSV antibodies for 1 h at 37° C. The cells were fixed in 1% formaldehyde and cell surface-bound antibody was detected by incubation with goat F(ab)2 anti-human IgG-PE conjugate (Beckman Coulter) for 30 min at 4° C. For the ELISA assay, HEp-2 cells were infected with either the RSV Long strain or the RSV 18537 strain (ATCC number VR-1580) in serum-free medium at a ratio of 0.01 pfu/cell.



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    Growth kinetics of parental TPP RSV-A2 and B1 and the derived MARMs. HEp-2 cells were inoculated at an MOI of 0.01 TCID 50 /cell. The cultured supernatant was sampled twice daily for four days and viral growth was assessed using a TCID 50 assay. RSV-A2 MARMs ( A-C ) and RSV-B1 MARMs ( D-F ) show similar growth to their parental TPP strains, with the exception of RSM-3 ( D ), which shows delayed and lower titre growth. MARMs, monoclonal antibody resistant mutants; MOI, multiplicity of infection; RSV, respiratory syncytial virus; TCID 50 , half-maximum tissue culture infective dose.

    Journal: bioRxiv

    Article Title: RSM01, an extended half-life RSV monoclonal antibody with a high barrier to resistance

    doi: 10.64898/2026.02.04.703721

    Figure Lengend Snippet: Growth kinetics of parental TPP RSV-A2 and B1 and the derived MARMs. HEp-2 cells were inoculated at an MOI of 0.01 TCID 50 /cell. The cultured supernatant was sampled twice daily for four days and viral growth was assessed using a TCID 50 assay. RSV-A2 MARMs ( A-C ) and RSV-B1 MARMs ( D-F ) show similar growth to their parental TPP strains, with the exception of RSM-3 ( D ), which shows delayed and lower titre growth. MARMs, monoclonal antibody resistant mutants; MOI, multiplicity of infection; RSV, respiratory syncytial virus; TCID 50 , half-maximum tissue culture infective dose.

    Article Snippet: Master stocks of RSV-A2 (ATCC VR-1540) and RSV-B1 (B WV/14617/85, VR-1400) were triple plaque purified in HEp-2 cells and propagated for a total of 5 and 8 passages, respectively, before use as parental strains.

    Techniques: Derivative Assay, Cell Culture, Infection, Virus